Automated detection of glaucoma eye and normal eye using color histogram method in MATLAB

I have eye fundus image of glaucoma eye and normal eye Step, 1. Input glaucoma or normal eye fundus image 2. Make automatic cropping systems only in the area of optical disc 3. Eliminating the blood vessels in the optical disc so that the measurement be accurate 4. Measuring the amount of area on the optical disc based on the pixels of image (for example, 1000x1000pixels) 5. Determining range pixels of glaucoma eye and normal eye based on the area of optical disc by pixels image (for example, glaucoma eye: 900x900pixels and normal eye 500x500pixels) 6. Create GUI to display all the steps and the results